Table 5.
Estimation of limit of detection (LOD) and limit of quantification (LOQ) of the CTX-like toxicity in fish flesh using the revisited CBA-N2a under non-destructive O/V treatment at 85.7/8.75 µM.
| Variability | Sample ID | ng P-CTX3C eq/mg Dry Extract | ng P-CTX3C eq/g Fish Flesh | ||
|---|---|---|---|---|---|
| LOD | LOQ | LOD | LOQ | ||
| Intra-assay ** | Cmic02 | 0.096 ± 0.006 | 0.200 ± 0.019 | 0.035 ± 0.002 | 0.072 ± 0.007 |
| Cmic19 | 0.041 ± 0.002 | 0.086 ± 0.008 | |||
| Emer05 | 0.030 ± 0.002 | 0.062 ± 0.006 | |||
| Emer13 | 0.026 ± 0.001 | 0.054 ± 0.005 | |||
| Inter-assay * | Cmic02 | 0.083 ± 0.024 | 0.173 ± 0.041 | 0.030 ± 0.008 | 0.062 ± 0.015 |
| Cmic19 | 0.035 ± 0.010 | 0.074 ± 0.018 | |||
| Emer05 | 0.026 ± 0.007 | 0.054 ± 0.013 | |||
| Emer13 | 0.022 ± 0.006 | 0.047 ± 0.011 | |||
* Data represent the mean ± SD of one microplate in three independent experiments (N2a cells at 795, 797 and 798 P). ** Data represent the mean ± SD of three microplates in one experiment (N2a cells at 810 P).