Table 6.
Dose-response curves parameters of CBA-N2a when detecting VGSC activators in fish samples.
| Variability | Sample ID | Top Absorbance | Bottom Absorbance | EC80 *** (pg/µL) | EC50 *** (pg/µL) | Hillslope |
|---|---|---|---|---|---|---|
| Intra-assay * | Cmic19 | 1.064 ± 0.007 | 0.001 ± 0.004 | 71.2 ± 8 | 130.6 ± 11 | −2.282 ± 0.132 |
| Emer05 | 1.110 ± 0.014 | 0.003 ± 0.004 | 114.7 ± 10 | 184.4 ± 10 | −2.927 ± 0.271 | |
| Inter-assay ** | Cmic19 | 1.084 ± 0.062 | −0.003 ± 0.002 | 55.9 ± 12.1 | 109.5 ± 21.1 | −2.056 ± 0.073 |
| Emer05 | 1.081 ± 0.101 | 0.000 ± 0.002 | 102.2 ± 15.9 | 171.3 ± 20.5 | −2.675 ± 0.196 |
* Data represent the mean ± SD of three microplates in one experiment (N2a cells at 810 P). ** Data represent the mean ± SD of one microplate in three independent experiments (N2a cells at 795, 797 and 798 P). *** OV+ conditions: 85.7/8.57 µM final concentrations.