Proximity of non-touching Oli-neu cells affects the thyroid hormone signaling pathway (modified from http://www.kegg.jp/pathway/mmu04919). (a) Significantly regulated genes. (b) Expression ratios and individual fold-change cut-offs (negative for down-regulation) of the significantly regulated genes. Note that Notch3 would be a false hit if the traditional 1.5× would be used instead of our individual gene cut-off because although the absolute expression ratio exceeded 1.5× it was below the individual gene CUT. (c) Gene Commanding Height (GCH) scores of selected TH genes. In addition to the significantly regulated TH genes, we plotted genes with high GCH scores in one condition: Na+/K+ transporting, alpha 3 polypeptide (Atp1a3), B cell leukemia/lymphoma 2 (BCL2)-associated agonist of cell death (Bad), caspase 9 (Casp9), subunits of mediator complex (Med1, Med16), notch 3 (Notch3), parathyroid hormone 1 receptor (Pth1r), regulators of calcineurin (Rcan1, Rcan2), and thyroid hormone receptor-associated protein 3 (Thrap3).