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. 2020 Apr 30;9(5):1114. doi: 10.3390/cells9051114

Figure 1.

Figure 1

Effects of Acute Insulin and IL-6 Treatment of SH-SY5Y Cells. SH-SY5Y cells were treated with 100nM of insulin, 10ng/mL of IL-6, or 20ng/mL of IL-6 for 30 min. (A) Acute insulin treatment significantly increases phosphorylation of Akt at the Serine 473 Site (n = 3 per group). (B) Acute IL-6 treatment significantly increases phosphorylation of STAT3 at Tyrosine 705 (n = 3 per group). (C) Acute insulin and IL-6 treatment significantly decreases phosphorylation of AMPK (n = 3 per group). (D) Acute insulin and IL-6 treatment significantly increases phosphorylation of AS160 (n = 3 per group). (E) Representative blots are shown beside the quantified data. Data are presented as means ± SE. A.U., arbitrary units. * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.001, as determined using a one-way ANOVA followed by Fisher’s LSD post hoc analysis.