Table 3.
IC50 and dose-ratios (DRs)-values of CCTX1 and CTX1B obtained from extracts with and without a cleanup step.
| CTXs Type |
Analysis Conditions |
IC50 ± SD (pg∙well−1) |
ng CTXs∙g−1 Fish ± SD | /Hill slope/ ± SD |
DR ± SD |
DRcorr ± SD |
IC50(corr) ± SD |
ng CTXs∙g−1 Fishcorr ± SD |
|---|---|---|---|---|---|---|---|---|
| CCTX1 | No matrix | 0.48 ± 0.04 | 0.024 ± 0.002 | 3.5 ± 0.5 | - | - | - | 0.024 ± 0.002 |
| Purified | 0.56 ± 0.04 | 0.027 ± 0.002 | 1.6 ± 0.1 | 1.1 ± 0.1 | 1.3 ± 0.1 | 0.6 ± 0.1 | 0.031 ± 0.003 | |
| Unpurified | 1.3 ± 0.1 | 0.06 ± 0.01 | 1.5 ± 0.3 | 2.6 ± 0.2 | 3.6 ± 0.9 | 1.8 ± 0.2 | 0.12 ± 0.02 | |
| CTX1B | No matrix | 0.26 ± 0.02 | 0.013 ± 0.001 | 2.4 ± 0.1 | - | - | - | 0.013 ± 0.001 |
| Purified | 0.27 ± 0.02 | 0.013 ± 0.001 | 2.4 ± 0.2 | 1.0 ± 0.1 | 1.0 ± 0.2 | 0.27 ± 0.05 | 0.014 ± 0.003 | |
| Unpurified | 0.34 ± 0.02 | 0.017 ± 0.001 | 2.3 ± 0.4 | 1.3 ± 0.1 | 1.6 ± 0.3 | 0.4 ± 0.1 | 0.022 ± 0.006 |