Figure 1.

T cell receptor (TCR) expression and reactivity of TCR DP04_chim redirected T cells. (A) Immunomagnetically selected and prestimulated human CD4 (left panels) and CD8 T cells (right panels) from an HLA-DPB1*04:01 negative healthy donor were transfected with TCR DP04_chim coding RNA (CD4 TCR DP04_chim and CD8 TCR DP04_chim) or without RNA (CD4 Mock and CD8 Mock) and analyzed after 16–20 h by flow cytometry for expression of CD4, CD8, as well as of TCR DP04_chim using TCR vβ 13.2 subfamily specific mAb. (B) IFN-γ spot formation and (C) cytolytic activity of TCR DP04_chim- and Mock-transfected CD4 and CD8 T cells upon incubation with HLA-DPB1*04:01 positive acute myeloid leukemia (AML) blasts from individual patients and EBV-LCL or, as controls with HLA-DPB1*04:01 negative target cells at an effector-to-target cell ratio (E:T) of (B) 0.1:1 or (C) as indicated. AML blasts in (B) were either left untreated or pretreated with 500 IU/mL IFN-γ for 24 h before testing. Standard deviation of mean is shown of two technical replicates. All experiments in Figure 1 are representative of one T cell donor out of three.