Figure 1.

Identification of the interaction of the human 293T cell proteins with Zika virus NS1 protein. (a) The identification of host factors binding to Zika virus NS1. Human 293T cells were transfected with c-Myc-tagged NS1-expressing vector. Twenty-four hours post transfection, the whole mock and NS1-transfected cell lysates were incubated with anti-c-Myc magnetic beads, then analyzed by SDS-PAGE, followed by staining with silver staining. The arrowheads indicate the position of NS1 protein and the band 1 and band 2 which are interacted with NS1 protein. (b) Co-immunoprecipitation of NS1 with CCT1-8. HA-tagged CCT1-8 proteins and naïve (nontagged) NS1 protein were expressed in 293T cells. After immunoprecipitation with anti-HA beads, samples were analyzed by SDS-PAGE, followed by immunoblotting using a HA tag or NS1-specific antibody.