. 2020 May 9;12(5):310. doi: 10.3390/toxins12050310

Table 2.

BMAA action on selected identified proteins based on the statistical significance of the observed differences in protein profile of Nostoc during nitrogen starvation.

No.	Protein	Gene	Function	Fold Regulation *	p-Value
No.	Protein	Gene	Function	LFQ Ratio BMAA Treated Sample/Control Sample	p-Value
Nitrogen metabolism (3 proteins)
1	nrtA	alr0608	nitrate-binding protein	1.76	0.002
2	glnB\|P-II	all2319	nitrogen regulatory protein	0.55	0.09
3	nifD	all1454	molybdenum-iron protein subunit alpha in nitrogenase	0.54	0.047
Heterocyst formation (two DNA-binding proteins)
4	Apb2	all1939	transcription regulation of hepA and hepC genes	0.63	0.08
5	Abp1	all1940	transcription regulation of hepA and hepC genes	1.49	0.078
Nucleotides metabolism, purine and pyrimidine (4 proteins)
6	EC:2.7.4.6 nucleoside diphosphate kinase	alr3402	transferring phosphorus-containing groups	0.75	0.004
7	EC:2.1.2.2 phosphoribosylglycinamide formyltransferase-2	alr1299	purine metabolism	2.14	0.05
8	EC:2.1.2.3 3.5.4.10 \| purH; bifunctional purine biosynthesis protein	all3093	purine metabolism	0.71	0.08
9	EC:1.1.1.205 inosine 5-monophosphate dehydrogenase	alr0051	purine metabolism	1.75	0.00013
Secondary metabolites (4 proteins)
10	EC:2.2.1.7 1-deoxy-xylulose 5-phosphate synthase	alr0599	Thiamine metabolism; Terpenoid backbone biosynthesis	found only in control sample	0.00001
11	EC:2.8.1.10 \| thiG thiazole synthase	all3519	Thiamine biosynthesis protein	found only in control sample	0.02
12	EC:1.17.7.1 1.17.7.3 \| ispG 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase	all2501	Terpenoid backbone biosynthesis	0.55	0.0012
13	EC:1.3.1.83 1.3.1.111 \| chlP geranylgeranyl hydrogenase	alr0128	Porphyrin and chlorophyll metabolism; Terpenoid backbone biosynthesis	0.49	0.01
Photosynthesis (9 proteins)
14	psaA photosystem I P700 chlorophyll a apoprotein A1	alr5154	photosystem I	0.38	0.01
15	petC cytochrome b6-f complex iron-sulfur subunit	all2453	cytochrome b6-f	1.75	0.024
16	psbW photosystem II 13kDa protein	all0801	photosystem II	found only in BMAA treated sample	0.04
17	petE plastocyanin	all0258	plastocyanin	0.58	0.06
18	cpcA phycocyanin alpha chain	alr0529	phycocyanin	0.56	0.075
19	apcD allophycocyanin B alpha chain	all3653	allophycocyanin	0.82	0.076
20	EC:4.2.1.24 delta-aminolevulinic acid dehydratase	alr4380	Porphyrin and chlorophyll metabolism	0.51	0.08
21	psaB photosystem I P700 chlorophyll a apoprotein A2	alr5155	photosystem I	0.26	0.08
22	psaF photosystem I subunit III	all0109	photosystem I	0.61	0.04
ABC-transporters and transporters (3 proteins)
23	branched-chain amino acid transport system substrate-binding protein	alr2535	ABC transporters; Quorum sensing	0.59	0.00003
24	secY \| preprotein translocase subunit	all4197	Quorum sensing	found only in control sample	0.03
25	peptide/nickel transport system substrate-binding protein	alr0140	periplasmic oligopeptide-binding ABC transporter Quorum sensing	1.61	0.038
Amino acids metabolism (8 protein)
26	EC 4.3.1.19 threonine dehydratase	alr4232	Glycine, serine and threonine metabolism; Valine, leucine and isoleucine biosynthesis	found only in control sample	0.0008
27	EC 2.6.1.52 phosphoserine aminotransferase	all1683	Glycine, serine and threonine metabolism; Cysteine and methionine metabolism	0.62	0.01
28	EC 2.5.1.47 cysteine synthase A	alr4416	Cysteine and methionine metabolism	0.69	0.03
29	EC 2.3.3.13 leuA 2-isopropylmalate synthase	alr4840	Valine, leucine and isoleucine biosynthesis; Pyruvate metabolism	0.55	0.06
30	ureC urease subunit alpha	alr3670	Arginine biosynthesis; Purine metabolism	0.82	0.08
31	EC:2.6.1.16 \| NodM glutamine--fructose-6-phosphate aminotransferase	alr3464	Alanine, aspartate and glutamate metabolism	2.20	0.002
32	EC:1.2.1.16 1.2.1.79 1.2.1.20 succinate-semialdehyde dehydrogenase / glutarate-semialdehyde dehydrogenase	all3556	Alanine, aspartate and glutamate metabolism; Lysine degradation	1.64	0.022
33	thrA homoserine dehydrogenase	all4120	Glycine, serine and threonine metabolism	1.73	0.05
Chaperones (3 proteins)
34	dnaK molecular chaperone DnaK	alr2990	Folding, sorting and degradation	found only in BMAA treated sample	0.002
35	dnaK molecular chaperone DnaK	alr1742	Folding, sorting and degradation	1.23	0.09
36	groES co-chaperonin GroES	alr3661	Chaperones and folding catalysts	1.54	0.08
Signalling, Stress response, GTP-binding proteins and proteases (8 proteins)
37	TypA	all4140	GTP-binding protein Ribosome –binding Stress responce	2.04	0.059
38	peroxiredoxin	alr4641	signaling and cellular processes; acting on a peroxide as acceptor	2.02	0.0015
39	EC:1.15.1.1 superoxide dismutase, Fe-Mn family	all0070	Acting on superoxide as acceptor	found only in BMAA treated sample	0.0028
40	EC:1.8.1.7 gor; glutathione reductase (NADPH)	all4968	Glutathione metabolism	1.45	0.047
41	EC:1.8.1.9 thioredoxin reductase	all0737	Selenocompound metabolism	2.22	0.07
42	AhpC/TSA family protein	alr4404	This family includes peroxiredoxin proteins	0.39	0.035
43	cyclic-di-GMP-binding protein	all4662	signaling and cellular processes	0.73	0.05
44	EC:3.4.24. \| ftsH cell division protease	all4936	cell division protease FtsH	1.89	0.015
Translation (4 proteins)
45	GTP-binding protein LepA	all2508	elongation factor	found only in control sample	0.05
46	EC:6.1.1.17 gltX \| glutamyl-tRNA synthetase	all3205	Aminoacyl-tRNA biosynthesis; Porphyrin and chlorophyll metabolism	found only in control sample	0.005
47	ribosome recycling factor (rrf)	alr1208	Translation factors	1.37	0.086
48	EC:6.1.1.6 lysS lysyl-tRNA synthetase	all4071	Aminoacyl-tRNA biosynthesis	1.28	0.096
Ribosomal proteins, (5 proteins)
49	rplX \| 50S ribosomal protein L24	asl4204	genetic information processing	2.56	0.014
50	rpsJ \| 30S ribosomal protein S10	all4336	genetic information processing	0.068	0.059
51	rpsF \| 30S ribosomal protein S6	all4802	genetic information processing	1.27	0.06
52	rps7 \| 30S ribosomal protein S7	all4339	genetic information processing	0.74	0.06
53	rplL \| 50S ribosomal protein L7/L12	alr5303	genetic information processing	0.67	0.094
Transcription (3 proteins)
54	EC:2.7.7.6 rpoB DNA-directed RNA polymerase subunit beta	alr1594	Transcription	1.52	0.05
56	EC:2.7.7.6 rpoC1 DNA-directed RNA polymerase subunit beta	alr1595	Transcription machinery	2.13	0.08
55	sigA \| RNA polymerase sigma factor RpoD	all5263	Transcription; Transcription regulation; DNA-binding	0.39	0.0046
DNA metabolism (4 proteins)
57	EC:5.6.2.2 DNA gyrase subunit A	all0860	DNA replication DNA repair and recombination	1.92	0.02
58	recA recombinase A	all3272	DNA repair and recombination	3.03	0.047
59	EC:5.6.2.2 gyrB DNA gyrase subunit B	all5265	Enzymes altering DNA conformation	1.56	0.08
60	nucA sugar-non-specific nuclease	all7362; alr8011	genetic information processing	2.63	0.08
CO₂ fixation and CO₂-concentrating mechanism (two proteins)
61	rbcL EC:4.1.1.39 ribulose bisophosphate carboxylase	alr1524	Carbon metabolism	0.67	0.0008
62	ccmM carbon dioxide concentrating mechanism protein	all0865	Carbon metabolism	0.64	0.02
Carbohydrate metabolism, Glycolisis and gluconeogenesis (6 proteins)
63	EC:2.7.2.3 phosphoglycerate kinase (pgk)	all4131	Glycolysis Gluconeogenesis	0.78	0.0008
64	nucleotide sugar epimerase	all3509	Sugar metabolism	0.59	0.07
65	EC:3.1.3.11 fructose-1,6-bisphosphatase I	all4021	Glycolysis Gluconeogenesis	0.76	0.006
66	EC:1.1.1.44 1.1.1.343 6-phosphogluconate dehydrogenase	alr5275	Pentose phosphate pathway, Glutathione metabolism	0.68	0.05
67	NADH-dependent butanol dehydrogenase	alr4566	Propanoate metabolism	0.73	0.05
68	Phosphoglucomutase	all5089	Glycogenolysis and Gluconeogenesis	0.77	0.057

* Fold regulation stands for BMAA/control ratio as it was done in [17], i.e., fold changes between BMAA-treated and control samples are shown (p < 0.1). Additional information about 164 identified BMAA-regulated proteins, whose fold regulation was detected with 0.1 < p < 1 (not significant) and with, N.A. i.e. not available value, because only in one or two sample replications the peptide was detected, thereby p-value could not be calculated, is presented in Tables S4 and S5, as a Supplementary.