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. 2020 Apr 29;9(5):1096. doi: 10.3390/cells9051096

Figure 5.

Figure 5

The sEH inhibitor TPPU is anti-inflammatory in coronary artery vascular smooth muscle cells. Expression of TNFα and CYP2J34 mRNA in (a) combined pig coronary and pulmonary artery vessels in organ culture at 24 h (n = 4), and (b) pig primary coronary artery cells at 4 h (CaSMCs) in the presence or absence of LPS (1 μg/mL). mRNA was measured by qRT-PCR and fold levels normalized to 18S. (c) Inflammatory target gene expression of TNFα, VCAM-1, ICAM-1, MCP-1 (CCL2) and CD40 in cultures of pCASMCs in the presence or absence LPS (1 μg/mL; 4 h), and/or sEH inhibitor TPPU (1 μM; given as a 1 h pretreatment before addition of LPS). * indicates p < 0.05 by paired t-test between cells treated with TPPU in the presence of absence of LPS. Data represents mean ± SE from n = 4 cultures from two separate animals.