Figure 4.

Immunocytochemical detection of neuronal subtypes in 3D neurospheres. Presence of synapses was determined with post-synaptic marker PSD95 and synaptic protein Synaptophysin (SYNP) double staining. Glutamatergic (VGLUT1/2), GABAergic (GAD65/67), cholinergic (VAChT) and dopaminergic (TH) neurons were detected in the developing 3D neurospheres from D28. All samples were stained with TUBB3 (in red) to label the neurites. Protein name IDs are indicated with colors, representing the color of the used fluorophore (e.g., green as Alexa 488; red as Alexa 594). Nuclei were counterstained with DAPI (in blue). Scale bar: 25 µm.