Table 13.
Overview on the extraction experiments carried out to investigate the influence of different solvents, at different temperatures on the extraction yield of PAs of Symphytum with PLE and the BfR-based extraction method. The result corresponds to the sum of lycopsamine and intermedine quantified by LC/MS/MS [122].
| Technique | Sample (g) | Volume (mL) | Time (min) | Temperature (°C) | Solvent | Result (ppm) | |
|---|---|---|---|---|---|---|---|
| 1 | PLE | 1 | 30 | 30 | 50/75/100/125 | Phosphoric acid 1% | 389.1/485.5/312.3/615.8 |
| 2 | PLE | 1 | 30 | 30 | 50/75/100/125 | Phosphoric acid 5% | 564.4/619.5/677.3/586.8 |
| 3 | PLE | 1 | 30 | 30 | 50/75/100/125 | Formic acid 1% | 321.3/318.4/422.6/462.6 |
| 4 | PLE | 1 | 30 | 30 | 50/75/100/125 | Formic acid 5% | 474.1/386.1/510.6/472.9 |
| 5 | PLE | 1 | 30 | 30 | 50/75/100/125 | Sulfuric acid 1% | 481.7/444.2/455.2/486.1 |
| 6 | PLE | 1 | 30 | 30 | 50/75/100/125 | Sulfuric acid 5% | 726.6/716.4/711.0/502.4 |
| 7 | BfR based | 2 | 40 | 30 | RT | Formic Acid/Methanol/Water | 251.7 |