Figure 8.

Dystrophin expression at mRNA and protein level. (A) Four weeks after the cellular therapy, mRNA for dystrophin gene expression in Patient 1 and Patient 3 was assessed at 20% (RQ = 0.206) and at 27% (RQ = 0.265), respectively, of healthy controls (*** p < 0.001). (B) In Patient 1, six months after the co-transplantation of BM-MSCs and myogenic stem/progenitor cells, about 15% of small myofibres expressed dystrophin (arrow). Myofibres expressing dystrophin (arrow) were present in the tissue samples taken from Patent 2 at 4 weeks after cellular therapy (EnVision^TM FLEX/HRP detection system staining with HRP magenta substrate chromogen).