Table 2.
Primers used in this study.
| Gene | Species | Sequence |
|---|---|---|
| GAPDH | Mouse | for: 5′-TGT TGA AGT CAC AGG AGA CAA CCT-3′ rev: 5′-AAC CTG CCA AGT ATG ATG ACA TCA-3′ |
| TGF-β1 | Mouse | for: 5′-ATA CGC CTG AGT GGC TGT CT -3′ rev: 5′-TCA TGG ATG GTG CCC AGG TC-3′ |
| Nrf2 | Mouse | for: 5′-CCG AGA TAT ACG CAG GAG AGG TA-3′ rev: 5′-GCT CGA CAA TGT TCT CCA GCT T-3′ |
| HO-1 | Mouse | for: 5′-TGG TGG CCC ACG CAT ATA C-3′ rev: 5′-CAT GGC CTT CTG TGC AAT CTT-3′ |
| IL-10 | Mouse | for: 5′-GAT GCC CCA GGC AGA GAA-3′ rev: 5′-CAC CCA GGG AAT TCA AAT GC-3′ |
| FoxP3 | Mouse | for: 5′-GGC AAA TGG AGT CTG CAA GTG-3′ rev: 5′-CAG GAG ATG ATC TGC TTG GCA-3′ |
| TNF-α | Mouse | for: 5′-AGC TCG TAG CAA ACC ACC AA-3′ rev: 5′-GAG AAC CTG GGA GTA GAC AAG G-3′ |
| IL-1β | Mouse | for: 5′-GGA GAA CCA AGC AAC GAC AAA-3′ rev: 5′-GGG TGT GCC GTC TTT CAT TAC-3′ |
| ADAM-17 | Mouse | for: 5′-CAC AAA ACT TGA GAG TCG TGG T-3′ rev: 5′-GCT AGA ACC CTA GAG TCA GGC-3′ |
| IL-6 | Mouse | for: 5′-CTG CAA GAG ACT TCC ATC CAG-3′ rev: 5′-AGT GGT ATA GAC AGG TCT GTT GG-3′ |
| Plin2 | Mouse | for: 5′-GTC CAC CTG ATT GAA TTC GC-3′ rev: 5′-CGA TGT GCT CAA CAC AGT G-3′ |
| G0S2 | Mouse | for: 5′-ACT GCA CCC TAG GCC CAG-3′ rev: 5′-GTC TCA ACT AGG CCG AGC AC-3′ |
| CD36 | Mouse | for: 5′-CAA ATG CAA AGA AGG AAA GCC-3′ rev: 5′-AAT GGT CCC AGT CTC ATT TAG C-3′ |
| Fabp1 | Mouse | for: 5′-GCA GAG CCA GGA GAA CTT TG-3′ rev: 5′-GGG TCC ATA GGT GAT GGT GAG- 3′ |
| β-Actin | Human | for: 5′-TCC ATC ATG AAG TGT GAC GT-3′ reverse: TAC TCC TGC TTG CTG ATC CAC-3′ |
| RPLP0 | Human | for: 5′-ACT GTG CCA GCC CAG AAC A-3′ rev: 5′-AGC CTG GAA AAA GGA GGT CTT C-3′ |
| CD62L | Human | for: 5′-GGA CTG CGT GGA GAT CTA TAT CAA-3′ rev: 5′-TGG CAG GCG TCA TCG TT-3′ |
| β7-Integrin | Human | for: 5′-GGA CTC CAG CAA CGT GGT ACA-3′ rev: 5′-TCA CGG TGG AAG ACA GGC TAT-3′ |
| MAdCAM-1 | Human | for: 5′-TGA GTG GCC AGC CTT TCC-3′ rev: 5′-CCC TGA CCA GTT CTC AAC TTG AA-3′ |
| ICAM-1 | Human | for: 5′-TGG CCC TCC ATA GAC ATG TGT-3′ rev: 5′-TGG CAT CCG TCA GGA AGT G-3′ |
| VCAM-1 | Human | for: 5′-CAA AGG CAG AGT ACG CAA ACA C-3′ rev: 5′-GCT GAC CAA GAC GGT TGT ATC TC-3′ |
Abbreviations used: ADAM-17, a disintegrin and metalloproteinase domain 17; CD36, cluster of differentiation 36; CD62L, Selectin L; Fabp1, fatty acid-binding protein 1; for, forward primer; FoxP3, Forkhead box P3; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; G0S2, Go(G1 switch gene 2; HO-1, heme oxygenase 1; ICAM-1, intercellular adhesion molecule 1; IL-1β, interleukin-1β; IL-10, interleukin-10; IL-6, interleukin-6; MAdCAM-1, Mucosal vascular addressin cell adhesion molecule; Nrf2, NEF2-related factor 2; Plin2, Perlipin 2; rev, reverse primer, RPLP0, ribosomal phosphoprotein large P0; TGF-β1, transforming growth factor-β1; TNF-α, tumour necrosis factor-α; VCAM-1, vascular cell adhesion protein 1.