Table 3.
Antibody panels used for flow cytometric analysis (FACS) analysis.
| Cross-Reactivity (Panel) | Antibody | Conjugate | Manufacturer | Cat. No. |
|---|---|---|---|---|
| Mouse (1) | CD45 | APC-Cy7 | BD Pharmingen, Heidelberg, Germany | 557659 |
| F4/80 | APC | eBioscience, Santa Clara, CA, USA | 47-4801 | |
| CD11b | PE | eBioscience | 12-0112-82 | |
| CD11c | PE-Cy7 | eBioscience | 25-0114-81 | |
| Ly6G/Ly6C | PerCP-Cy5.5 | Biolegend, San Diego, CA, USA | 127615 | |
| Ly6G | FITC | eBioscience | 17-5931-81 | |
| Hoechst 33258 | - | Sigma Aldrich, Merck, Taufkirchen, Germany | B2883 | |
| Mouse (2) | CD45 | APC-Cy7 | BD Pharmingen | 557659 |
| CD3 | PE-Cy7 | Biolegend | 100219 | |
| CD4 | FITC | eBioscience | 11-0042-82 | |
| CTLA4 | PE | eBioscience | 12-1522-81 | |
| CD8 | PerCP-Cy5.5 | BD Pharmingen | 551162 | |
| CD25 | PE | eBioscience | 12-0025-81 | |
| FoxP3 | APC | eBioscience | 17-5773-80 | |
| Hoechst 33258 | - | Sigma Aldrich | B2883 | |
| Human (1) | CD45 | BV510 | BD Pharmingen | 563204 |
| CD4 | APC-Cy7 | BD Pharmingen | 561839 | |
| CD8 | AF700 | BD Pharmingen | 557945 | |
| CD62L | PE-Cy7 | BD Pharmingen | 565535 | |
| CD66b | APC | eBioscience | 17-0666-42 | |
| CD14 | VioGreen | Milteny Biotech, Bergisch Gladbach, Germany | 130-096-875 | |
| CD68 | PE-eFluor610 | eBioscience | 61-0689-41 | |
| Hoechst 33258 | - | Sigma Aldrich | B2883 |
Abbreviations used are APC, Allophycocyanin; BV510, brilliant violet 510; CD, cluster of differentiation, FITC, Fluorescein isothiocyanate; Ly6G/Ly6C, lymphocyte antigen 6 complex, PE, Phycoerythrin.