Table 4.
Composition of buffers used for primary liver sinusoid endothelial cell isolation.
| Chemical | SC1 | SC2 | GBSS-A | GBSS-B |
|---|---|---|---|---|
| NaCl | 8000 mg/L | 8000 mg/L | - | 8000 mg/L |
| KCl | 400 mg/L | 400 mg/L | 370 mg/L | 370 mg/L |
| NaH2PO4 × H2O | 88.17 mg/L | 88.17 mg/L | - | - |
| Na2HPO4 | 120.45 mg/L | 120.45 mg/L | 59.6 mg/L | 59.6 mg/L |
| HEPES | 2380 mg/L | 2380 mg/L | - | - |
| NaHCO3 | 350 mg/L | 350 mg/L | 227 mg/L | 227 mg/L |
| EGTA | 190 mg/L | - | - | - |
| Glucose | 900 mg/L | - | 991 mg/L | 991 mg/L |
| CaCl2 × 2H2O | - | 560 mg/L | 225 mg/L | 225 mg/L |
| MgCl2 × 6H2O | - | - | 210 mg/L | 210 mg/L |
| MgSO4 × 7H2O | - | - | 70 mg/L | 70 mg/L |
| KH2PO4 | - | - | 30 mg/L | 30 mg/L |
Abbreviations used are: EGTA, ethylene glycol bis(beta-aminoethyl ether)-N,N,N′,N′-tetraacetic acid; GBSS, Gey’s balanced salt solution; HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.