Table 3.
Primers used for qRT-PCR, protein expression and promoter regions of tent and tetR for EMSA experiments.
| Target Gene | Primer | Nucleotide Sequence (5′-->3) | Product Length (bp) |
|---|---|---|---|
| qRT-PCR | |||
| tent | P1714-F | CCAAGGTGCACAAGGAATTT | 146 |
| P1715-R | CAATGTTTAATGCGGGTCCT | ||
| tetR | P1726-F | GTTGCTCAAATTATTTAAACTTCGAA | 115 |
| P1727-R | GCTATATCACATTCTTTCATATCTTCAAA | ||
| rpoB | P2142-F | TTGAAGAATGTAAAGAGAGAGATGCTAC | 118 |
| P2143-R | GGGAAGTCACCCATAAAGACA | ||
| gyrA | P2146-F | AAGATGATGTAGCAGTAAGTATGGA | 98 |
| P2147-R | CTCTGAAGCCAATGTCCTTTT | ||
| Recombinant protein expression | |||
| CTC_p21 | P2349-F | CGCCGCGGATCCATGTATAAGATATTGATTGTTGAA | 711 |
| P2350-R | CCGCCGGAATTCTTACACCTGAAATAAACGATAGCC | ||
| CTC_01979 | P2351-F | CGCCGCGGATCCATGAACAAAATAAATTGTGTAATAATA | 792 |
| P2352-R | CCGCCGGAATTCTTAAAAATCTAATATGTCCTTTAAGTG | ||
| CTC_01421 | P2353-F | CGCCGCGGATCCGTGAACAACATATTGTTAGTTGAA | 717 |
| P2354-R | CCGCCGGAATTCCTATTTATTAATTTCGTAGTTCCACCT | ||
| codY | P2355-F | CGCGGATCCATGTCATCATTATTAGAGAAG | 801 |
| P2356-R | CCGCCGGAATTCTTACTTAATTTTTTTCAATTCCTC | ||
| CTC_00935 | P2357-F | CGCGGATCCGTGTGTAGAGTAGTGCTT | 759 |
| P2358-R | CCGCCGGAATTCTTATACTTTTTTATTATTCAC | ||
| EMSA | |||
| Ptent | P2365-F | (5’-end labelled biotin) GGTGGCTCCATCATAATAATTGTAT | 359 |
| P2366-R | (5’-end labelled biotin) GGTTTTAGCATTAAAAAAATTAGAACCTA | ||
| PtetR | P2363-F | (5’-end labelled biotin) CAGTATTTTTGAAATGTATAATAATTACTTC | 316 |
| P2364-R | (5’-end labelled biotin) CGGTTCTCTTAATTTAGTAATATCAATAT | ||
F, forward; R, reverse.