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. 2020 May 22;12(5):567. doi: 10.3390/v12050567

Figure 3.

Figure 3

Phylogeny of TMUV-TP1906, Tembusu virus strains (TMUVs) and duck Tembusu virus strains (DTMUVs) based on the ORF (10,278 nt). The evolutionary history was inferred by using the maximum likelihood method based on the Tamura–Nei model [22]. The tree with the highest log likelihood is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the maximum composite likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 40 nucleotide sequences. All positions containing gaps and missing data were eliminated. Evolutionary analyses were conducted in MEGA7 [21]. The reliability of the analysis was calculated using 1000 bootstrap replication. Bootstrap support values >75 are shown. The scale bar indicates nucleotide substitutions per site.