. 2020 May 22;12(5):567. doi: 10.3390/v12050567

Table 1.

Primers used in RT-PCR and DNA sequencing for TMUV-TP1906.

Name	Sequence (5′ to 3′)	Amplicon (bp)	Annealing Temp (°C)	References
P1f	AGAAGTTCRYCTGTGTGA	638	50	[6,18]
DF_R638	CAGCAGTCTATGTCTTCAGG	638	50	[6,18]
DF_F441	CGATAGTTGCTGGGCTGAAGC	675	50	[6]
DF_R1115	GCAGTAAGATCTCACAACCGC	675	50
DF_F954	GCTTCAGCTGTCTGGGGATGC	696	50
DF_R1650	CAATGACTCTTTGTTTTGCCACG	696	50
DF_F2353	GGCACTGCTATTGTGGATGGG	987	50
DF_R3339	GGTGGGGTGGTGCAAGACC	987	50
DF_F3302	GGAACAACTGTCACAGTAACG	1393	50
DF_R4694	GCATGACTCCCACTCCAGCC	1393	50
DF_F4406	GCATCACAGAGATTTGATGTGG	757	54
DF_R5162	CCTGAACCTGGATGTAGGTCC	757	54
DF_F4874	GCAAGTCATCGTCGTGCAACC	709	50
DF_R5582	GCTCTTCAATGTCTGTTATTGGC	709	50
DF_F5399	GCTCACACCTCAGCGAGTGC	851	50
DF_R6249	GGTCATTGTAACTTATCCCAGC	851	50
DF_F6494	CGCTCACAGAATGACAGAATCC	855	50
DF_R7348	GGAACATCTGTAGCCACTATGC	855	50
DF_F6807	GAACCAGAGAGACAGAGATCGC	1352	50
DF_R8158	CCCTAGCTAGCCATTCCTCGG	1352	50
DF_F7940	GCAGGTTCAGGAAGTGAGAGG	597	50
DF_R8536	GGATTGTCTTGGTCATAATGCC	597	50
DF_F8383	GGATGCACAAAACCAACCGC	833	50
DF_R9215	GGCCGAGATGTCACGCAGC	833	50
DF_F9274	GGGACACTAGAATAACCAAGGC	1212	50
DF_R10485	CCAACATCCGGTGGCAGGG	1212	50
TMUV-E_F	TTCAGCTGTCTGGGGATGCA	1503	50	[19]
TMUV-E_R	GGCATTGACATTTACTGCCA	1503	50
TMUV-NS1_F	GACACGGGGTGCTCAATCGACTT	1056	50
TMUV-NS1_R	AGCCATGACCTTTGATTTGAT	1056	50
TMUV-NS3_F	GGAGGAGTCATCTGGGATGTG	1857	50
TMUV-NS3_R	TCTCTTTCCACTCGCAAAATC	1857	50
TMUV-NS5_F	GAACTGGCAGAACTTTGGGGGAG	2711	50
TMUV-NS5_R	TTACAAGACACCTTCACTCCAGC	2711	50
1_F	AAATGACTTCAGGACACCTC	723	50	This study *
1_R	ACATACCTTGTCCACACTTC	723	50
2_F	ATGTCATGGATCACTCAAGG	400	50
2_R	CAGTCAAGTCAATGCTGTTG	400	50
3_F	AAAAGAAAGGAGGCATGCTA	523	50
3_R	GGAACATCCCATATGACTCC	523	50
5_F	CAGTCGGAAGTGCATTAAAC	683	54
5_R	CAGCTGTAGTCAGCATGTAT	683	54
7_F	TTAGAATCCTGTCAAAGCCC	767	50
7_R	CACCTTCACCACCTTATTCA	767	50
8_F	CTGGAATCTCGTTGATAGGG	572	50
8_R	TAATGAGTTGAACGCACAGA	572	50
3′UTR-2_F	CCAACCCTCAATAGGTTCAA	612	50	This study ^†
3′UTR-2_R	GAGGGTCTCCTAGTCTATCC	612	50
3′UTR-5_F	ATACATGGAAGACAAGACCC	465	50
3′UTR-5_R	GAGACGGTATTGAACGCTTA	465	50
3′UTR-10_F	AGGAGCTAAGCGTTCAATAC	427	50
3′UTR-10_R	GACTCTGTGTTCTACCACC	427	50
GSP-5′ end	GGTCGCCTCACTGACCCCAACTAGC	331	55	For RACE
3′UTR-10_F	AGGAGCTAAGCGTTCAATAC	424	55
oligo d(T)-anchor	GACCACGCGTATCGATGTCGACT(16)V		55

* Design based on Sitiawan virus cDNA sequence (JX477686); ^† design based on M1775 virus cDNA sequence (MH414569).