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AF546-Phal does not cross the membranes of ATDC5 cells in the absence of lysenin. (A) Transmitted light image of cells exposed to AF546-Phal and calcein-AM. (B) The lack of red fluorescence indicates that AF546-Phal did not cross the membranes not exposed to lysenin. (C) The strong green fluorescence recorded after over five hours indicates that the cells remained viable. (D) The overlap of transmitted and green channel images shows calcein distribution inside cells.
