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. 2020 Apr 27;9(5):1083. doi: 10.3390/cells9051083

Figure 2.

Figure 2

Covalent binders target tubulin for degradation. (A) Structural formulae of T007-1 and T138067. (B) TUBA and TUBB protein levels in hTert-RPE1 cells upon treatment with T007-1 and T138067. (C) Quantification of tubulin protein levels across the indicated conditions. All data are normalized to reference protein (ACTB) and tubulin protein levels in DMSO-treated cells. Lines represent the average of three biological replicates. Error bars represent standard error of the mean (SEM). (D) Biochemical partitioning of tubulin into soluble (S) and polymerized (P) forms across the indicated conditions. Note that both the S and the P fraction of tubulin decrease upon treatment with 1 μM T007-1 and T138067 for 5 h.