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. 2020 Apr 27;9(5):1083. doi: 10.3390/cells9051083

Figure 4.

Figure 4

Tubulin evades CA4-CRBN targeted degradation. (A) The shortest linker docking pose of CRBN-bound lenalidomide (PDB ID 5fqd) and tubulin-bound CA4 (PDB ID 5lyj). (B) Corresponding shortest pairwise distance between lenalidomide and CA4 is permissive of a degrader approach. (C) Chemical synthesis of CA4-CRBN a degrader compounds with varying linkers. (D) TUBA and TUBB protein levels in hTert-RPE1 cells upon treatment with the indicated compounds. (E) Quantification of tubulin protein levels across the indicated conditions. All data are normalized to ACTB, then to tubulin protein levels in DMSO-treated cells. Lines represent average of three biological replicates. Error bars represent SEM.