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. 2020 Apr 25;9(5):1068. doi: 10.3390/cells9051068

Figure 2.

Figure 2

The effect of dexamethasone on HMGB1 release in primary cultured cortical astrocytes. (A) Increasing concentrations of dexamethasone induced HMGB1 release from astrocytes. Cells were treated with the indicated concentrations of dexamethasone for 24 h, and the amount of HMGB1 in the media was analyzed by Western blotting. Representative blots are shown (HMGB1: 30 kDa). The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01 vs. basal (concentration “0”) (Dunnett’s test; n = 4–10). (B) Astrocytes were treated with 100 nM dexamethasone for the indicated periods of time and the amount of HMGB1 in the media was analyzed by Western blotting. Representative blots are shown (HMGB1: 30 kDa). The data are expressed as the mean ± SEM. * p < 0.05 vs. basal (time “0”) (Dunnett’s test; n = 5–12).