Figure 5.

Functionality of the A_2A-NMDA receptor heteromer in the APP_Sw,Ind mice model of Alzheimer’s disease(AD). In (A–L), the microglial and neuronal primary cultures from the hippocampus of the WT and APP_Sw,Ind mice (E,H) were pre-incubated with vehicle, 1 μM of the A_2AR antagonist SCH-58261 (SCH) or 1 µM of the NMDAR antagonist, MK-801 (MK), followed by treatment with 100 nM of the A_2AR agonist CGS-21680 (CGS), 15 µM NMDA or both, and the cAMP levels (A–D), ERK 1/2 phosphorylation signal (E–H) and the representative traces of dynamic mass redistribution (DMR) over time (I–L) were determined. Values are the mean ± SEM of 10 to 12 different experiments. ERK 1/2 phosphorylation levels and cAMP increases are expressed as percentage over basal. A one-way ANOVA followed by a Bonferroni multiple comparison post-hoc test showed a significant effect over basal (* p < 0.05, ** p < 0.01, *** p < 0.001).