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. 2020 May 11;11(5):534. doi: 10.3390/genes11050534

Table 1.

Numbers of mtDNA variants detected in pairwise comparisons of HT-29 cells according to the following thresholds: depth of 30 reads and minimum allele frequency of 3%.

Protocol All Common Hom Het %Common Unique Confirmed %Confirmed
DOP 41 37 31 6 90.24 4 33 89.19
eWGA 44 40 37 3 90.91 4 36 90
MALBAC 3 3 2 1 100 0 2 66.67
MDA 43 38 36 2 87.37 5 36 94.74

Protocol: amplification protocol; All: all variants detected in each pair; Common: number of variants shared by each pair; Hom: number of homoplasmic variants; Het: number of heteroplasmic variants; %Common: percentage of variants shared by each pair; Unique: number of private variants; Confirmed: number of variants confirmed by the bulk; %Confirmed: percentage of variants confirmed by the bulk.