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. 2020 May 13;9(5):1208. doi: 10.3390/cells9051208

Figure 2.

Figure 2

Immunofluorescence of SNX27 and AQP2 in HeLa cells. FLAG-tagged SNX27 and HA-tagged AQP2 constructs were transiently transfected into HeLa cells and immunolabeling of FLAG and HA was done. A negative control study revealed no immunolabeling of FLAG and HA in the HeLa cells transiently transfected with SNX27-full length and HA-tagged AQP2 constructs, which were incubated only with secondary antibody (omitting the incubation of primary antibodies) (A–C). SNX27 (SNX27-Full Length, Δ(PX+FERM), and ΔFERM) and AQP2 were co-localized throughout the cytoplasm in HeLa cells (D–L). In contrast, when the PDZ domain was deleted in SNX27 (SNX27-ΔPDZ), SNX27 and AQP2 accumulated in a punctate pattern, and more eccentrically localized in the perinuclear region of the HeLa cells (M–O). (P) Co-localization of SNX and AQP2 was analyzed by calculation of the Pearson’s coefficient. Graphs express means ± SE (>20 cells per group; three independent experiments). Scale bars, 10 μm.