Figure 5.
Immunofluorescence microscopy of AQP2 and SNX27 in primary cultured inner medullary collecting duct (IMCD) cells of the rat kidney. The immunofluorescence labeling of AQP2 and SNX27 in vehicle-treated IMCD cells ((A,B) Control), dDAVP (10−9 M)-treated IMCD cells for 24 h ((D,E) dDAVP), and IMCD cells with dDAVP withdrawal for 3 h after dDAVP (10−9 M)-treatment for 24 h ((G,H) dDAVP-3 h). Immunofluorescence labeling of AQP2 and SXN27 was merged (C,F,I). (J) Co-localization of AQP2 and SNX27 was analyzed by calculation of the Pearson’s coefficient. Graphs express means ± SE (>200 cells per group; two independent experiments). * p < 0.05. Scale bars, 10 μm.