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. 2020 May 13;9(5):1208. doi: 10.3390/cells9051208

Figure 6.

Figure 6

Semiquantitative immunoblotting and quantitative real-time PCR of SNX27 and AQP2 in mpkCCDc14 cells with siRNA-mediated SNX27 knockdown. (A–C) Semiquantitative immunoblotting of SNX27 (~65 kDa) and AQP2 (~29 kDa and ~35–50 kDa) in total cell lysates from mpkCCDc14 cells treated with vehicle or dDAVP (10−9 M) for 24 h under control siRNA or SNX27-siRNA transfection. n indicates the number of cell preparations from three independent experiments. (D,E) SNX27 and AQP2 mRNA level in mpkCCDc14 cells treated with vehicle or dDAVP (10−9 M) for 24 h under control siRNA or SNX27-siRNA transfection. n indicates the number of cell preparations from two independent experiments. * p < 0.05.