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. 2020 May 13;9(5):1208. doi: 10.3390/cells9051208

Figure 7.

Figure 7

Semiquantitative immunoblotting of SNX27 (A,B; F,G; and K,L) and AQP2 (A,C; F,H; and K,M) in total cell lysates from mpkCCDc14 cells transfected with control-siRNA or SNX27-siRNA. Semiquantitative immunoblotting of AQP2 in total cell lysates from mpkCCDc14 cells transfected with control siRNA or SNX27-siRNA subjected to 24-h dDAVP stimulation (10−9 M) or 3-h withdrawal (Withdrawal) after dDAVP stimulation (10−9 M, 24 h) in the absence (-) or the presence (+) of chloroquine (10−4 M, for the last 3 h, (A,D,E)), bafilomycin (10−7 M, for the last 3 h, (F,I,J)), or MG-132 treatment (MG-132, 10−6 M, for the last 3 h, (K,N,O)). n indicates the number of cell preparations from three independent experiments. D, dDAVP. * p < 0.05.