Skip to main content
. 2020 May 13;9(5):1208. doi: 10.3390/cells9051208

Figure 8.

Figure 8

Cell surface biotinylation assay of AQP2 in mpkCCDc14 cells with siRNA-mediated SNX27 knockdown under dDAVP stimulation. (A–C) Semiquantitative immunoblotting of SNX27 (~65 kDa) and AQP2 (~29 kDa and ~35–50 kDa) in the total cell lysates or biotinylated fraction from mpkCCDc14 cells transfected with control siRNA or SNX27-siRNA. (A,D) Cell surface biotinylation assay for examining the changes in dDAVP (10−9 M, 24 h)-induced AQP2 expression in the apical plasma membrane of the mpkCCDc14 cells. (E) Immunofluorescence microscopy of AQP2 in mpkCCDc14 transfected with control siRNA or SNX27-siRNA, followed by dDAVP treatment (10−9 M, 24 h). In the x-z images, arrows indicate AQP2 expression in the apical plasma membrane. n indicates the number of cell preparations from three independent experiments. * p < 0.05 when compared to control siRNA-transfected cells.