Table 1.
Primer sequences.
| Construct | pGEX-4T-1 Vector Primer Sequence (5′-3′) | p3XFLAG-CMV-10 Vector Primer Sequence (5′-3′) | |
|---|---|---|---|
| SNX-Full Length | F | GGTTCCGCGTGGATCCATGGCGGACGAGGACGGG | TGACGATGACAAGCTTATGGCGGACGAGGACGGG |
| R | GATGCGGCCGCTCGAGCTAGGTGGCCACATCCCT | TCGCGGCCGCAAGCTTCTAGGTGGCCACATCCCT | |
| SNX27-Δ(PX+FERM) | F | GGTTCCGCGTGGATCCATGGCGGACGAGGACGGG | TGACGATGACAAGCTTATGGCGGACGAGGACGGG |
| R | GATGCGGCCGCTCGAGCTATGTGTAATCATAAAATGATTG | TCGCGGCCGCAAGCTTCTATGTGTAATCATAAAATGATTGTCCCAAG | |
| SNX27-ΔFERM | F | GGTTCCGCGTGGATCCATGGCGGACGAGGACGGG | TGACGATGACAAGCTTATGGCGGACGAGGACGGG |
| R | GATGCGGCCGCTCGAGCTAATTCTCATCAGATTCTGACAG | TCGCGGCCGCAAGCTTCTAATTCTCATCAGATTCTGACAGGAAC | |
| SNX27-ΔPDZ | F | GGTTCCGCGTGGATCCAAGCAAGCAGTGCCCATA | TGACGATGACAAGCTTATGAAGCAAGCAGTGCCCATA |
| R | GATGCGGCCGCTCGAGCTAGGTGGCCACATCCCT | TCGCGGCCGCAAGCTTCTAGGTGGCCACATCCCT |