Figure 3.

ALKBH5 regulated AURKB expression in RCC cells and correlated with AURKB expression in human RCC tissues. (A,B) AURKB expression was obviously decreased in ALKBH5-knockdown cells and increased in ALKBH5 overexpression cells as revealed by western blotting. (C) IHC analysis of ALKBH5 and AURKB in RCC at 200× magnification. Scale bars indicate 100 µm. Yellow staining or brown staining indicates positive. (D) A moderate positive correlation between the expression of ALKBH5 and AURKB mRNAs was found in 26 paired RCC tissues. (E) Kaplan-Meier survival curves showed that AURKB upregulation was significantly associated with poor overall survival in RCC patients. (F) IHC analysis of AURKB and Ki67 in xenografts. The histogram indicates the AURKB and Ki67-positive cells from the panel. Yellow staining or brown staining indicates positive. *, P<0.05. RCC, renal cell carcinoma; IHC, immunohistochemistry.