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. 2020 May;8(10):646. doi: 10.21037/atm-20-3079

Figure 6.

Figure 6

HIF induced by hypoxia upregulated ALKBH5 expressions in RCC. (A) ALKBH5 and AURKB mRNAs were upregulated in the 1% O2 group as compared with 20% O2 group as revealed by qRT-PCR. Caki-1 and 786-0 cells were exposed to 20% or 1% O2. RNA was extracted at 24 h. Data are presented as mean ± SD from 3 independent experiments; *, P<0.05. (B) HIF-1α. HIF-2α, ALKBH5, and AURKB proteins were up-regulated in the 1% O2 group as compared to the 20% O2 group as revealed by western blot. Caki-1 and 786-0 cells were exposed to 20% or 1% O2. Protein was extracted at 48 h. (C) Working model: ALKBH5 promoted RCC cell proliferation by stabilizing AURKB mRNAs in an m6A-dependent manner. RCC, renal cell carcinoma; qRT-PCR, real-time quantitative polymerase chain reaction.