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. 2020 Apr 30;9(5):1109. doi: 10.3390/cells9051109

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Silencing of PARP1 does not affect cell fusion or expression of fusion genes. (A) Successful PARP1 silencing by siRNA is shown by immunoblotting of C2C12 cells after four days of differentiation. (B) Myotubes were analyzed by eMHC immunofluorescence in si Ctrl versus si PARP1 conditions at day 4. Nuclear DNA was stained by DAPI. (C) Percentage of myotube distribution divided into three groups, according to the range of nuclei per myotube. Data in C are the median of 600 myotubes from four distinct areas from three biological replicates, * p < 0.05, Student’s t-test. (D) Relative mRNA levels of five fusion genes (identified in Figure 3) in si Ctrl and si PARP1 conditions. Data in D are the mean of four independent experiments + SD; * p < 0.05; Student’s t-test.