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. 2020 Apr 30;9(5):1109. doi: 10.3390/cells9051109

Figure 5.

Figure 5

MacroH2A1.1 (mH2A1.1) occupies fusion-related genes in myotubes. (A) Heatmaps and summary plots using normalized read coverages from mH2A1.1 ChIP-seq from myotubes after four days of differentiation. Signals over the body of all genes +/- 8 kb are shown. Heatmaps were divided into three categories, according to normalized read counts: not expressed, lowly expressed and highly expressed genes. Fusion genes of interest belong to the category of highly expressed genes. (B) Pathway analysis of the 46 genes of interest by Paintomics3 analysis. (C) Snapshots from the UCSC genome for macroH2A1.1, total macroH2A1 and the enhancer mark histone H3K4me2 in proliferating myoblasts in growth medium (GM) or myotubes in differentiation medium (DM) and input are shown. The exact coordinates are indicated in Table A1. (D) Levels of macroH2A1.1 enrichment by ChIP-qPCR on Fn1, Itga11 and Col1a1 in proliferative (GM) and differentiated (DM, four days) cells. As a background control, we used IgG. Data are mean of n = 3 independent experiments, normalized to the input signal, + SD; * p < 0.05; Student’s t-test. (E) Levels of macroH2A1.1 enrichment by ChIP-qPCR on three loci of Col1a1 genes, in si ctrl, si macroH2A1.1 and si macroH2A1.2 conditions at day 4. As a background control, we used IgG. Data are the mean of n = 4 independent experiments, normalized to the input signal, + SD; * p < 0.05; Student’s t-test.