Figure 12.

Inhibition of both proteasome and autophagy promotes cell death. MO3.13 cells treated without/with psychosine (Psy) for 72 hs concomitant with MG132 (MG) and chloroquine (CQ) in the last 24 h. (a–c) Levels of ROS (a), basal respiration (b), and cell viability (c) were quantified. Values areexpressed as mean ± S.D. (ROS, n = 3; Respiration, n = 5; Viability, n = 6). Statistical significance *p < 0.05, ***p < 0.001 in comparison to control (C). C/MG/CQ vs. Psy, or Psy/MG/CQ, # p < 0.05, ### p < 0.001. Psy vs. Psy/MG/CQ, +++ p < 0.001.