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. 2020 May 6;12(5):514. doi: 10.3390/v12050514

Figure 2.

Figure 2

Serum antibody responses against DENV2 induced after TC or ID immunizations. DENV-specific serum antibody responses induced after TC and ID 3rd immunization doses, measured by ELISA with concentrated DENV particles as solid phase (n = 5). (A,B) Serum IgG subclass responses in mice immunized via TC (A) or ID (B) routes. Values indicated above the lines represent IgG1/IgG2a ratio, measured in each immunized group. (C,D) Binding of anti-DENV2 antibodies from serum samples of immunized mice via TC (C) and ID (D) routes to intact and heat-denatured virus particles. (E,F) Antigen binding affinity of anti-DENV2 antibodies induced after immunization via TC (E) or ID (F) routes. Serum samples from mice submitted to TC (E) or ID (F) immunizations were tested by ELISA using different concentrations of ammonium thiocyanate as a dissociating agent. Statistical analyses were performed by two-way ANOVA in association with Bonferroni’s post-test * p < 0.05, **** p < 0.0001. Representative results of 2 independent experiments.