Figure 2.

Bone marrow stromal cells producing a soluble IGF-IR inhibit experimental hepatic metastasis of lung and colon carcinoma. Syngeneic female C57Bl/6 (A and C) or nude (B and D) mice were implanted with 10⁷ genetically engineered marrow-derived stromal cells (MSCs) expressing sIGFIR (MSC^sIGFIR) or control MSC (MSC^GFP) embedded in Matrigel. Fourteen days later (A–D), the mice were inoculated via the intrasplenic/portal route with 10⁵ H-59 (A and B), 5 × 10⁴ murine colon carcinoma MC-38 (C) or 10⁶ human colon carcinoma KM12SM (D) cells. Mice were euthanized and liver metastases enumerated 14–16 (A), 18 (C) or 21 (D) days after or imaged using the IVIS 100 Xenogen 15 days (B) post tumor inoculation. Shown in (A) are the pooled data of three and in (B–D) individual experiments. Results of optical imaging are shown in (B). ** p < 0.01, *** p < 0.001, as determined by the non-parametric Mann–Whitney test. Reproduced from [89].