Figure 5.

Role of miR modulation in eNOS protein expression in hCAECs treated with the vasculotoxic stimuli, LPS. (A) Relative concentration of miR-17-5p, miR-92a-3p, miR-126-3p, miR-145-5p, miR-221-3p, and miR-222-3p in hCAECs according to LPS concentration (n = 3). (B) Densitometry of eNOS after transfection of each anti-miR-17-5p, anti-miR-92a-3p, anti-miR-126-3p, anti-miR-145-5p, anti-miR-221-3p, and anti-miR-22-3p (n = 4). One-way analysis of variance was applied and data are presented as mean ± standard deviation. * Indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the control group with LPS treatment. eNOS, endothelial nitric oxide synthase; hCAECs, human coronary artery endothelial cell; LPS, lipopolysaccharide; miR, microRNA; ns, not significant