Figure 2.

TurboID-fusion proteins biotinylate without the addition of biotin at similar levels to BioID- fusion proteins with biotin supplementation. Whole-cell lysates of A549 cells stably expressing BioID- or TurboID-tagged LaA, Nup43, or Nup53 fusion proteins were analyzed via western blot in the presence (+) of 50 μM biotin supplementation for 18 h or absence (−) of biotin supplementation. Anti-HA was used to detect fusion protein levels and streptavidin-HRP was used to probe for biotinylation. Anti-tubulin was used as a protein loading control.