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. 2020 May 25;9(5):1319. doi: 10.3390/cells9051319

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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In vitro and in vivo CM protection by puromycin pretreatment after ischemia/reperfusion. (A) Experimental design representing ischemia-like hypoxia/reoxygenation (H/R) protocols achieved in isolated adult mouse CM. (B) Scatter plots showing mortality of CM subjected to H/R (Ctrl H/R) or CM concomitantly subjected to H/R and a 200 µM puromycin pretreatment (puro H/R). Evaluation of CM mortality was assessed via propidium iodide (PI) staining. (C) Experimental design showing the I/R protocols performed in mice by a blind test comparing different concentration of puromycin. (D) Scatter plots representing individual I/R mouse by the percentage of necrosis area (AN) over area-at-risk (AR). n = number of animals. Statistics: + p < 0.05, ++ p < 0.01 vs. Ctrl H/R. (E) Representative images of Evan’s blue- and triphenyltetrazolium chloride (TTC)-stained hearts from Ctrl I/R and Puro I/R mice.