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. 2020 May 6;9(5):1142. doi: 10.3390/cells9051142

Figure 1.

Figure 1

p62 interacts with BECN1 proteins. (A) HEK293T cells were transfected with mock control vector, Flag-tagged p62, or Myc-tagged BECN1, as indicated. Transfected cells were harvested, and cell lysates were immunoprecipitated with anti-Flag antibody and probed with anti-Myc or anti-Flag antibody. (B) HEK293T cells were transfected with mock vector, Flag-tagged p62, or Myc-tagged BECN1 WT and Myc-tagged BECN1 truncated mutants. Transfected cells were harvested, and cell lysates were immunoprecipitated with anti-Myc antibody. Immunoprecipitated complexes were separated by SDS-PAGE, and probed with anti-Myc or anti-Flag antibody. (C) A schematic view of the molecular interaction between BECN1 and p62.