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. 2020 Apr 29;9(5):1101. doi: 10.3390/cells9051101

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of SIRT4. (A) SIRT4 protein was immunostained with a specific antibody in normal human liver tissue, cancer adjacent liver tissue, and chronic hepatitis and cirrhosis tissue samples and observed under microscopy at 400× magnification. In comparison with normal liver tissues, chronic hepatitis and cirrhosis tissues exhibited lower expression levels of SIRT4. (B) Immunoreaction scoring of SIRT4 between human liver tissue samples (cancer adjacent liver tissue, chronic hepatitis and cirrhosis) and normal liver tissue samples. The values are mean ± S.D. Statistical analysis was performed using Kruskal–Wallis test for single comparisons. * p < 0.05, *** p < 0.001. The cancer adjacent liver, chronic hepatitis and cirrhosis were compared with the adjacent normal liver.