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. 2020 May 24;9(5):1304. doi: 10.3390/cells9051304

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The detection of miR-299-3p, miR-150-5p, and miR-145-5p in hiPSCs and perinatal SCs and their relationship with the protein expression of NANOG, OCT4, and SOX2. The graph shows the (a) miR-145-5p expression, (b) miR-150-5p, and (c) miR-299-3p (primary y axis) and the expression of the NANOG, OCT4, and SOX2 proteins, as measured with the mean fluorescence intensity by the immunocytometry analysis (secondary y axis) in SCs from different fetal annexes. The miRNAs were detected by real time PCR, the fold change was determined from the −ΔΔCt values, calculated using U6 as reference gene and normalized to hiPSCs, as the control condition. The graphs show the mean ± SD of 5 independent experiments, p < 0.05 with hiPSCs (*), hAFSCs (#), and hFM-MSCs (§).

The detection of miR-299-3p, miR-150-5p, and miR-145-5p in hiPSCs and perinatal SCs and their relationship with the protein expression of NANOG, OCT4, and SOX2. The graph shows the (a) miR-145-5p expression, (b) miR-150-5p, and (c) miR-299-3p (primary y axis) and the expression of the NANOG, OCT4, and SOX2 proteins, as measured with the mean fluorescence intensity by the immunocytometry analysis (secondary y axis) in SCs from different fetal annexes. The miRNAs were detected by real time PCR, the fold change was determined from the −ΔΔCt values, calculated using U6 as reference gene and normalized to hiPSCs, as the control condition. The graphs show the mean ± SD of 5 independent experiments, p < 0.05 with hiPSCs (*), hAFSCs (#), and hFM-MSCs (§).