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. 2020 May 1;9(5):1123. doi: 10.3390/cells9051123

Figure 5.

Figure 5

The effect of CDDO-Me on NFκB-S276 phosphorylation in microglia following SE. SE increases NFκB-S276 phosphorylation in the FPC without altering its expression level, which is ameliorated by CDDO-Me. NFκB-S276 phosphorylation is increased in IB4 microglia following SE, while it is rarely observed in control animals. CDDO-Me abolishes NFκB-S276 phosphorylation in IB4 microglia following SE. (A) Representative Western blot image for NFκB and NFκB-S276. (B) Quantification of the effect of CDDO-Me on NFκB-S276 phosphorylation based on Western blot data. Error bars indicate SEM (*,# p < 0.05 vs. control and vehicle, respectively; n = 7, respectively). (C) Representative images for NFκB-S276 phosphorylation in IB4 microglia. (D,E) Quantification of the effect of CDDO-Me on the number of NFκB-S276 positive cells (D) and NFκB-S276 signals (E) following SE. Open circles indicate each value. Horizontal bars indicate the mean value. Error bars indicate SEM (* p < 0.05 vs. vehicle; n = 7, respectively).