Figure 3.

Seven-day 100 µM H₂O₂ treatment increased senescence in hNDFs but did not affect contractile protein expression. (A) hNDFs were stained for P21, Calponin, and α-SMA. P21 expression was higher in hNDFs treated with 100 µM H₂O₂. Scale bars represent 100 µm. (B) Quantification of p21, calponin, and α-SMA immunofluorescent staining. Seven-day treatment with 100 µM H2O2 caused a significant increase in hNDF senescence compared to 0 µM controls (# p < 0.001). Expression of calponin and α-SMA was not affected by 100 µM H2O2. N = 3. Bars represent mean ± SEM.