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. 2020 May 13;9(5):1207. doi: 10.3390/cells9051207

Figure 5.

Figure 5

Cell seeding efficiency and the effect of fiber diameter size on oAECs’ proliferation. Cells were seeded on Petri dishes (oAECs) or ha1-PLGA and ha2-PLGA electrospun fleeces and allowed to grow for up to 48 h. (A) DNA quantification by Qubit® dsDNA HS Assay on oAECs on Petri dishes or seeded fleeces after 4 h and 48 h of culture to verify cell adhesion, seeding efficiency, and to assess DNA quantity. It is shown that there is a higher proliferation rate in the case of ha2-PLGA compared to ha1-PLGA after 48 h of culture. *** and **** Statistically significant in the same sample type at different time points (p < 0.001 and p < 0.0001, respectively). °°°° Statistically significant between different sample types at 48 h (p < 0.0001) (n = 3 for each type of sample/analysis/time point, fleece size: 15 mm × 7 mm). (B) Comparison showing that increasing pore size of ha-PLGA fleeces is accompanied by an increase in cell proliferation rate confirmed by a significant increase of DNA quantity in ha2-PLGA compared to ha1-PLGA (p < 0.0001) after 48 h of culture.