Skip to main content
. 2020 May 9;9(5):1183. doi: 10.3390/cells9051183

Figure 2.

Figure 2

C70PLY4 targets TLR4 to inhibit the LPS-activated transendothelial migration of PMNs isolated from TLR4-wild-type mice. PMNs were isolated from C3H/HeN (TLR4-wild-type) and C3H/HeJ (TLR4-deficient) mice. The isolated PMNs were treated with PBS to serve as a control or with various PLY fragments, including PLY, PLY4, and C70PLY4, and then the isolated PMNs were added to the Transwell upper chamber cultured with HUVECs. LPS (1 μg/mL) was added to the lower chamber to attract the transendothelial migration of the PMNs. The capability of PMN transendothelial migration was determined by collecting the PMNs in the lower chamber. *, p < 0.05 vs. TLR4-wild-type PMNs without LPS priming. #, p < 0.05 vs. TLR4-wild-type PMNs treated with LPS and PLY.