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. 2020 Apr 29;11(5):485. doi: 10.3390/genes11050485

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Verification of the VviGT14 promoter activity in tobacco leaf. A. Diagram of main cis-acting elements in the VviGT14 promoter region. B. Transient expression of the VviGT14 promoter-driven luciferase (LUC) gene; tobacco leaves were transformed with equal amount of the proVviGT14::LUC construct (left side of the leaf) and empty LUC construct (right side of the leaf). Right panel: fluorescence imaging. Left panel: relative expression of the LUC gene in the experimental and control groups. The data are expressed as the mean ± SD from at least six biological replicates. ANOVA tests (**, P < 0.01).