Figure 7.

Regulation of VviWRKY40 on the promoter activity of VviGT14 in a transient expression system (A, B and C) and the expression of the related genes in the VviWRKY40 over-expressed tobacco leaves (D). A. The reporter, effector and normalized control constructs used in the dual luciferase assay. B. The suppression of VviWRKY40 on the VviGT14 promoter activity in a transient expression system of Arabidopsis protoplasts using dual luciferase assay. The control represents pART7 without any transcription factors. The data are displayed as the mean ± SD from at least six biological replicates. ANOVA tests (*, P < 0.05). C. The suppression of VviWRKY40 on the VviGT14 promoter activity in a transient expression system in Vitis vinifera L. “Chardonnay” cell suspension using dual luciferase assay. VviMYBA2 (BAD18978), a specific transcription factor in anthocyanin biosynthesis, is taken as negative control to test the reliability of this reaction system. The data are expressed as the mean ± SD from at least six biological replicates. D. Relative expression of NbGT and VviWRKY40 in transiently over-expressed tobacco leaves, EV, as a control, indicates empty-vector expressed tobacco leaves; OE-VviWRKY40 indicates VviWRKY40 overexpressed tobacco leaves; The data were shown as the mean ± SD from at least three biological replicates. ANOVA tests (*, P < 0.05. **, P < 0.01). E. The boxplots of the concentrations of free-form (left) and glycosylated linalool (medium) in the Vv-WRKY40 transiently overexpressed and control tobacco leaves as well as the ratio of glycosylated linalool to the total (right). At least three data are contained in a boxplot. At least three biological replicates were performed.