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. 2020 Apr 28;9(5):1093. doi: 10.3390/cells9051093

Figure 1.

Figure 1

Effect of ONX 0914 on the manifestation of chronic viral myocarditis in NMRI mice. (A) Mice were inoculated with 5 × 105 pfu of Coxsackievirus B3 strain 31-1-93. Between days 3 and 8, mice were treated with ONX 0914 (5–10 mg/kg) or vehicle (Captisol) daily—thereafter treatment continued three times per week until day 28 (vehicle n = 20; ONX 0914 n = 20). (B) Body weight was monitored as indicated and analyzed using two-way ANOVA. (C) Mice were sacrificed after 28 days. RNA was extracted from heart tissue and Coxsackievirus B3 (CVB3) genome expression was determined by quantitative PCR. Paraffin-embedded, hematoxylin-eosin (D) and Masson’s trichrome-stained cardiac tissue (E) was taken for histological scoring of cardiac injury and inflammation. (F) Two representative images (upper panel from the group treated with 5–7 mg/kg bodyweight ONX 0914; lower panel from the group treated with 10 mg/kg bodyweight ONX 0914) are shown for each treatment group. Data are mean ± SEM and analyzed by unpaired t-tests. p-values are indicated in each graph.